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In a further embodiment, the present invention is directed toward a modified TetR(E) protein comprising an amino acid substitution at a position selected from the group consisting of positions 59, 70, 71, 91, 95, 96, 99, 100, 101, 103, 114, 127, 158, 159, 160, 175, 189, 193, and 197 of the TetR(E) protein as depicted in SEQ ID NO: 42, wherein said modified TetR(E) protein binds a TetR(E) operator sequence with greater affinity in the presence of tetracycline than in the absence of tetracycline! Doxycycline half-life was decreased from 153 hours to 111 hours!
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Strain from infant 120, and the dotted arrows show the changed position of one band in the strain from infant 123!
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